Abstract |
Recently, interleukin-4 (IL-4) has been clarified as having T-cell growth factor activity; therefore, it becomes of interest whether IL-4, as well as interleukin-2 (IL-2), affects the proliferation of leukemic cells derived from mature T cells. In the present study, we describe a case of chronic T-lymphocytic leukemia (T-CLL) with monoclonal proliferation of human T-lymphotropic retrovirus (HTLV)-I or HTLV-II negative CD3(+)4(+)8(-) T cell expressing IL-2 receptors without stimulation. Radiolabeled IL-2 binding assay revealed 750 high-affinity and 6,750 low-affinity binding sites per cell. In accordance with the expression of high-affinity IL-2 receptors, the leukemic cells proliferated in response to exogenous IL-2 without prior stimulation. In addition, exogenous IL-4 also induced their proliferation. Moreover, IL-2 and IL-4 exerted a synergistic effect on the leukemic cell proliferation. Although the expression of IL-2 or IL-4 mRNA was not detected in fresh leukemic cells, the expression of IL-2 mRNA, but not IL-4 mRNA, was induced by phytohemagglutinin stimulation, and the leukemic cells proliferated. These findings suggest that not only IL-2, but also IL-4 are involved in the proliferation of leukemic cells of T-CLL.
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Authors | H Umadome, T Uchiyama, R Onishi, T Hori, H Uchino, N Nesumi |
Journal | Blood
(Blood)
Vol. 72
Issue 4
Pg. 1177-81
(Oct 1988)
ISSN: 0006-4971 [Print] United States |
PMID | 3262382
(Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-2
- Interleukins
- RNA, Messenger
- Receptors, Interleukin-2
- Interleukin-4
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Topics |
- Humans
- Interleukin-2
(metabolism, pharmacology)
- Interleukin-4
- Interleukins
(metabolism, pharmacology)
- Leukemia, Prolymphocytic, T-Cell
(immunology, metabolism)
- Lymphocyte Activation
(drug effects)
- Male
- Middle Aged
- Phenotype
- RNA, Messenger
(biosynthesis)
- Receptors, Interleukin-2
(isolation & purification)
- T-Lymphocytes
(classification, immunology, metabolism)
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