Abstract |
We have compared sequencing of cloned "polymerase chain reaction" (PCR) products and the direct sequencing of PCR products in the examination of individuals from six families affected with alpha 1-antitrypsin (AAT) deficiency. In families where paternity was in question we confirmed consanguinity by DNA fingerprinting using a panel of locus-specific minisatellite probes. We demonstrate that direct sequencing of PCR amplification products is the method of choice for the absolutely specific diagnosis of AAT deficiency and can distinguish normals, heterozygotes and homozygotes in a single, rapid and facile assay. Furthermore, we demonstrate the reproducibility of the PCR and a rapid DNA isolation procedure. We have also shown that two loci can be simultaneously amplified and that the PCR product from each locus can be independently examined by direct DNA sequencing.
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Authors | C R Newton, N Kalsheker, A Graham, S Powell, A Gammack, J Riley, A F Markham |
Journal | Nucleic acids research
(Nucleic Acids Res)
Vol. 16
Issue 17
Pg. 8233-43
(Sep 12 1988)
ISSN: 0305-1048 [Print] England |
PMID | 3262215
(Publication Type: Journal Article)
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Chemical References |
- DNA, Satellite
- alpha 1-Antitrypsin
- DNA
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Topics |
- Base Sequence
- DNA
(genetics)
- DNA, Satellite
(genetics)
- Female
- Gene Amplification
- Genes
- Genotype
- Humans
- Male
- Molecular Sequence Data
- Pedigree
- alpha 1-Antitrypsin
(genetics)
- alpha 1-Antitrypsin Deficiency
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