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Human immunodeficiency virus infection of CD4-bearing cells occurs by a pH-independent mechanism.

Abstract
The effect of weak bases (NH4Cl and amantadine) and carboxylic ionophores (monensin) on the infection of CD4 (T4) positive human cell lines by HIV-1 is examined. These reagents, which raise the pH of acidic intracellular organelles, fail to inhibit HIV-1 entry and the events leading to viral protein synthesis at concentrations inhibitory for low pH-dependent fusogenic enveloped viruses. The infectivity of VSV (HIV-1) pseudotypes is unaffected by weak bases at concentrations causing 95% plaque reduction of VSV in its own envelope. HIV-1 dependent cell--cell fusion (syncytium formation) occurs in medium maintained at pH 7.4-7.6, and virions are not irreversibly inactivated by incubation in acid medium. Our results show that HIV-1 entry and membrane fusion do not require exposure to low pH. The production of infectious HIV-1 particles, however, is inhibited in cells treated with NH4Cl.
AuthorsM O McClure, M Marsh, R A Weiss
JournalThe EMBO journal (EMBO J) Vol. 7 Issue 2 Pg. 513-8 (Feb 1988) ISSN: 0261-4189 [Print] England
PMID3259178 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Differentiation, T-Lymphocyte
  • Viral Envelope Proteins
Topics
  • Antigens, Differentiation, T-Lymphocyte (genetics)
  • Cell Line
  • Cell Transformation, Viral
  • HIV (genetics, pathogenicity)
  • Humans
  • Hydrogen-Ion Concentration
  • Viral Envelope Proteins (biosynthesis, physiology)

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