Abstract |
We describe a new ELISA which allows the measurement of the concentration of venom antigens in whole blood. The assay can be performed in less than 20 min and requires a 200 microliters sample of blood. It allows the accurate evaluation of concentrations of Vipera ammodytes venom in quantities smaller than 1 ng/ml of blood. Using this ELISA, we were able to follow in rabbits the kinetics of experimental envenomation with non-lethal doses of venom. This ELISA was also used to measure post mortem the level of venom antigens in various tissues such as liver, kidney, muscles and abdominal serosity of a rabbit. The method, which might be adapted to measure envenomation by other snake species, seems to be sufficiently rapid and sensitive to allow routine evaluation of the gravity of a snake bite in humans and to estimate the efficacy of immunotherapy.
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Authors | H Labrousse, A K Nishikawa, C Bon, S Avrameas |
Journal | Toxicon : official journal of the International Society on Toxinology
(Toxicon)
Vol. 26
Issue 12
Pg. 1157-67
( 1988)
ISSN: 0041-0101 [Print] England |
PMID | 3238700
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animals
- Antigens
(analysis)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Immunization, Passive
- Kinetics
- Male
- Mice
- Rabbits
- Snake Bites
(diagnosis, therapy)
- Snake Venoms
(analysis, immunology)
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