Abstract |
Murine RCT(+) sarcoma cells were sorted using a fluorescence-activated cell sorter with regard to the expression of H-2 antigens and then an increased H-2-expressing subclone was established, and named RCT(+)H-2+. The experimental metastasis of RCT(+) cells was compared with that of RCT(+)H-2+ cells by counting pulmonary colonies on the 21st day after i.v. inoculation of tumor cells (5-10 x 10(4)/mouse). When mice were inoculated with RCT(+) cells, mean numbers of pulmonary colonies were 2.1(range 0-6), 2.8(range 0-7) using 5 x 10(4) and 1 x 10(5) cells, respectively. On the other hand, in the mice inoculated with RCT(+)H-2+ cells, figures obtained were 7.0(range 4-16), 31.9(range 13-79), using 5 x 10(4) and 1 x 10(5) cells, respectively. The survival rate of RCT(+)H-2+ cells was higher than that of RCT(+) cells, when this was assayed in the early stage after i.v. injection of 51Cr-labeled cells (1 x 10(5) cells/mouse). In addition, RCT(+)H-2+ cells were more resistant than RCT(+) cells to lysis mediated by natural killer cells. These data suggest that an increase in metastatic ability was paralleled by an increase in the H-2 antigen expression and a decrease in sensitivity to the natural killer cells.
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Authors | K Masuyama, H Ochiai, S Ishizawa, K Tazawa, S Niwayama, M Fujimaki |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 114
Issue 5
Pg. 487-92
( 1988)
ISSN: 0171-5216 [Print] Germany |
PMID | 3182907
(Publication Type: Journal Article)
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Chemical References |
- Antigens, Neoplasm
- H-2 Antigens
- Histocompatibility Antigens
- tumor-associated transplantation antigen
- Picibanil
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Topics |
- Animals
- Antigens, Neoplasm
(analysis)
- Cytotoxicity, Immunologic
- H-2 Antigens
(analysis)
- Histocompatibility Antigens
(analysis)
- Killer Cells, Natural
(immunology)
- Lung Neoplasms
(secondary)
- Male
- Mice
- Mice, Inbred C3H
- Picibanil
(pharmacology)
- Sarcoma, Experimental
(immunology)
- Spleen
(immunology)
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