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Relation of H-2 expression on murine RCT(+) sarcoma cells to lung colonization and sensitivity to NK cells.

Abstract
Murine RCT(+) sarcoma cells were sorted using a fluorescence-activated cell sorter with regard to the expression of H-2 antigens and then an increased H-2-expressing subclone was established, and named RCT(+)H-2+. The experimental metastasis of RCT(+) cells was compared with that of RCT(+)H-2+ cells by counting pulmonary colonies on the 21st day after i.v. inoculation of tumor cells (5-10 x 10(4)/mouse). When mice were inoculated with RCT(+) cells, mean numbers of pulmonary colonies were 2.1(range 0-6), 2.8(range 0-7) using 5 x 10(4) and 1 x 10(5) cells, respectively. On the other hand, in the mice inoculated with RCT(+)H-2+ cells, figures obtained were 7.0(range 4-16), 31.9(range 13-79), using 5 x 10(4) and 1 x 10(5) cells, respectively. The survival rate of RCT(+)H-2+ cells was higher than that of RCT(+) cells, when this was assayed in the early stage after i.v. injection of 51Cr-labeled cells (1 x 10(5) cells/mouse). In addition, RCT(+)H-2+ cells were more resistant than RCT(+) cells to lysis mediated by natural killer cells. These data suggest that an increase in metastatic ability was paralleled by an increase in the H-2 antigen expression and a decrease in sensitivity to the natural killer cells.
AuthorsK Masuyama, H Ochiai, S Ishizawa, K Tazawa, S Niwayama, M Fujimaki
JournalJournal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 114 Issue 5 Pg. 487-92 ( 1988) ISSN: 0171-5216 [Print] Germany
PMID3182907 (Publication Type: Journal Article)
Chemical References
  • Antigens, Neoplasm
  • H-2 Antigens
  • Histocompatibility Antigens
  • tumor-associated transplantation antigen
  • Picibanil
Topics
  • Animals
  • Antigens, Neoplasm (analysis)
  • Cytotoxicity, Immunologic
  • H-2 Antigens (analysis)
  • Histocompatibility Antigens (analysis)
  • Killer Cells, Natural (immunology)
  • Lung Neoplasms (secondary)
  • Male
  • Mice
  • Mice, Inbred C3H
  • Picibanil (pharmacology)
  • Sarcoma, Experimental (immunology)
  • Spleen (immunology)

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