In vitro study of Fc-receptor function in autoimmune diseases.

Abstract

A simple test for studying in vitro Fc-receptor function of mononuclear phagocytes is described. Immune phagocytosis is analyzed as a dynamic phenomenon by using nearly pure suspensions of monocytes incubated for diverse times with autologous erythrocytes sensitized with highly purified IgG. In a series of normal volunteers and patients with vasculitis a strict correlation has been found between this in vitro assay and the measure of splenic clearance of IgG-coated red blood cells (RBC), the classical approach for studying in vivo macrophage Fc-receptor function by using sodium chromate 51Cr as tag. The use of this in vitro assay appears to be valuable mainly in cases requiring repeated measurements of Fc-receptor function for monitoring the course of disease or the effects of therapy.

• Authors: D Roccatello, J C Bensa, R Coppo, C Rollino, M Diaz, G Martina, G Piccoli
• Journal: Immunology letters (Immunol Lett) Vol. 9 Issue 1 Pg. 53-6 ( 1985) ISSN: 0165-2478 [Print] Netherlands
• PMID: 3157644 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Chromium Radioisotopes
• Immunoglobulin G
• Receptors, Fc
• Receptors, IgG

Topics

• Autoimmune Diseases (immunology)
• Chromium Radioisotopes
• Erythrocyte Aging
• Erythrocytes (physiology)
• Humans
• Immunoglobulin G (metabolism)
• Monocytes (immunology, metabolism)
• Phagocytosis
• Receptors, Fc (physiology)
• Receptors, IgG