Specific lipoxygenase inhibition reverses macrophage cytotasis towards P815 tumor cells in vitro induced by the calcium ionophore A23187.

Abstract

A23187-stimulated cytostatic activity of peritoneal macrophages towards P815 tumor cells served as a model for macrophage activation: a macrophage enriched preparation, separated on the basis of cell size in a discontinuous FCS gradient column, expressed cytostatic activity when stimulated by A23187. This was inhibited dose-dependently, by AA-861 but not by nordihydroguaiaretic acid (NDGA). AA-861 inhibited 5-lipoxygenase specifically, NDGA inhibited both 5-lipoxygenase- and cyclooxygenase activity. The ratio cyclooxygenase/lipoxygenase products increased with AA-861 but not with NDGA. These results show that lipoxygenase products are necessary for expression of cytostatic activity of these arachidonic acid metabolite-producing macrophages and that the ratio cyclooxygenase/lipoxygenase metabolites plays an important role in macrophage activation.

• Authors: J A Van Hilten, G R Elliott, I L Bonta
• Journal: Prostaglandins, leukotrienes, and essential fatty acids (Prostaglandins Leukot Essent Fatty Acids) Vol. 34 Issue 3 Pg. 187-92 (Dec 1988) ISSN: 0952-3278 [Print] Scotland
• PMID: 3146755 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzoquinones
• Lipoxygenase Inhibitors
• Quinones
• Calcimycin
• Masoprocol
• 2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone
• Arachidonate Lipoxygenases

Topics

• Animals
• Arachidonate Lipoxygenases (antagonists & inhibitors)
• Benzoquinones
• Calcimycin (pharmacology)
• Cytotoxicity, Immunologic
• Female
• Lipoxygenase Inhibitors
• Macrophages (enzymology, immunology)
• Masoprocol (pharmacology)
• Mice
• Mice, Inbred BALB C
• Neoplasms, Experimental (immunology)
• Quinones (pharmacology)