Dispersed pancreatic endocrine cells (PECs) were recently shown to survive indefinitely in the brain of allogeneic diabetic rat recipients across the major histocompatibility barrier without immunosuppression. Purified PECs (2-3 X 10(6) cells) prepared from Wistar rat islets were transplanted in 10 different sites in
streptozocin-induced diabetic ACI rats.
Blood glucose and
body weight changes were monitored throughout the study. PEC isografts (n = 5) and allografts (n = 6) were nonfunctional when transplanted in intramuscular, intravenous, and intrahepatic sites. When transplanted intraportally (n = 6) and intraperitoneally (n = 6), similar grafts were functional but had a short survival period (6.6 +/- 1.5 and 15.3 +/- 16.6 days, respectively). Prolonged graft survival in some recipients was observed when kidney
capsule [5, 9, 10, 240, 282, and 300 (2 rats) days], omentum pocket (7, 8, 10, 90, 105, 154, 155, and 157 days), and testis (7, 8, 12, 150, 230, and 234 days) were the
transplantation sites. Permanent graft survival was achieved in 20 of 20 recipients with intracerebral
transplantation and 21 of 22 recipients with intrathecal
transplantation. These findings confirm that dispersed single PECs can be transplanted as a permanent functional graft and can normalize the
hyperglycemia of the diabetic recipients. The duration of PEC graft survival is variable and depends on the
transplantation site. Both the cerebral cortex and subarachnoid space, which are immunologically privileged sites, have provided the best allograft protection.