Abstract |
A 78-kD protein (p78) is induced in human cells in response to interferon (IFN). It appeared as a radioactive spot when newly synthesized proteins from IFN-treated human cells labeled with [35S] methionine were separated in a two-dimensional system and autoradiographed. p78 was induced by IFN-alpha in normal human fibroblasts, and in some, but not all, established human tumor cell lines. It has been purified to homogeneity from Namalwa cells induced by recombinant IFN-alpha. Mouse polyclonal and monoclonal antibodies specific to p78 have been produced which allowed its quantitative determination in a Western blot ELISA. Using this method it was also shown that although IFN-gamma was a poor inducer of p78, it markedly increased the effect of IFN-alpha on p78 induction and accumulation. It was also demonstrated that p78 and the protein Mx of influenza-resistant mice, which we purified and characterized earlier (Horisberger and Hochkeppel, J. Biol. Chem. 260, 1730-1733, 1985) share common properties such as size, pI, amino acid composition, antigenic determinant(s), and IFN inducibility.
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Authors | M A Horisberger, H K Hochkeppel |
Journal | Journal of interferon research
(J Interferon Res)
Vol. 7
Issue 4
Pg. 331-43
(Aug 1987)
ISSN: 0197-8357 [Print] United States |
PMID | 3117906
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Interferon Type I
- Mx1 protein, mouse
- Myxovirus Resistance Proteins
- Proteins
- Interferon-gamma
- GTP-Binding Proteins
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Topics |
- Animals
- Antibodies, Monoclonal
- Cell Line
- Drug Synergism
- GTP-Binding Proteins
- Humans
- Interferon Type I
(pharmacology)
- Interferon-gamma
(pharmacology)
- Mice
- Molecular Weight
- Myxovirus Resistance Proteins
- Protein Biosynthesis
- Proteins
(immunology, isolation & purification)
- Species Specificity
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