Antibodies to nuclear lamins in autoimmune liver disease.

Abstract

Antibodies to nuclear lamins were detected in sera of patients with autoimmune liver disease. In indirect immunofluorescence tests, these sera revealed staining of the nuclear periphery. Using isolated nuclei, nuclear matrices, nuclear lamina-pore complexes, and chromatographically purified lamins as antigen source, the nuclear lamins A, B, and C were identified as reactive antigens in immunoblotting experiments. The lamins were also identified by 2-D gel electrophoresis. Antibodies to nuclear lamins occurred in 12 of 16 cases of active lupoid hepatitis, but not in 35 patients with the disease in remission. However, only 3 of 37 sera of patients with primary biliary cirrhosis contained anti-lamin antibodies. Autoimmune liver disease sera reacted preferentially with lamins A/C and less frequently with lamin B or lamins A/B/C.

• Authors: J Wesierska-Gadek, E Penner, E Hitchman, G Sauermann
• Journal: Clinical immunology and immunopathology (Clin Immunol Immunopathol) Vol. 49 Issue 1 Pg. 107-15 (Oct 1988) ISSN: 0090-1229 [Print] United States
• PMID: 3044653 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Lamin Type B
• Lamins
• Nuclear Proteins

Topics

• Antibodies (analysis)
• Autoimmune Diseases (immunology)
• Electrophoresis, Polyacrylamide Gel (methods)
• Fluorescent Antibody Technique
• Hepatitis, Chronic (etiology, immunology)
• Humans
• Lamin Type B
• Lamins
• Liver Cirrhosis, Biliary (immunology)
• Liver Diseases (immunology)
• Lupus Erythematosus, Systemic (complications)
• Nuclear Proteins (immunology)