Abstract |
An enzyme-linked immunosorbent assay (ELISA) was established for the rapid detection of specific antibodies against the causative agent of border disease in ovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified bovine virus diarrhea virus was used as test antigen. The optimal amount of antigen was 0.5 microgram/well, and the optimal concentration of conjugate was at 1/4,000 dilution. A total of 20 ovine serum samples, which had been collected from animals with or without border disease, were compared by ELISA and serum neutralization test for the detection of border disease-specific antibodies. ELISA was shown to be equally specific but less time-consuming and easier to perform than serum neutralization test. A positive correlation (r = 0.60) between the two tests was found.
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Authors | H J Chu, M M Sawyer, C A Anderson, R J Higgins, Y C Zee |
Journal | Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
(Can J Vet Res)
Vol. 51
Issue 2
Pg. 281-3
(Apr 1987)
ISSN: 0830-9000 [Print] Canada |
PMID | 3038292
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antibodies, Viral
- Antigens, Viral
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Topics |
- Animals
- Antibodies, Viral
(analysis)
- Antigens, Viral
(immunology)
- Border Disease
(diagnosis, immunology)
- Diarrhea Viruses, Bovine Viral
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Neutralization Tests
- Pestivirus
(immunology)
- Sheep
- Sheep Diseases
(immunology)
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