Trigeminal ganglion infection by thymidine kinase-negative mutants of herpes simplex virus after in vivo complementation.

Abstract

Infection of trigeminal ganglion by herpes simplex virus (HSV) thymidine kinase-negative (TK-) mutants was investigated in mixed infection studies in mice. Mice were corneally inoculated with TK- HSV alone or with mixtures of TK- HSV-TK+ HSV. When inoculated alone, an arabinosylthymine-selected HSV type 1 TK- mutant and a HSV type 2 TK- deletion mutant infected mouse ocular tissues but rarely infected ganglion tissues. However, both TK- mutants readily infected ganglion tissues when they were inoculated in mixtures with TK+ HSV. By means of mixed infection studies, it was demonstrated that TK- HSV could readily establish acute and latent ganglion infections. It was thought that the frequent infection of trigeminal ganglion tissue by both TK- mutants after mixed TK(-)-TK+ HSV infection was the result of in vivo complementation. After mixed TK(-)-TK+ HSV infection and subsequent cultivation of ganglion explants in arabinosylthymine, results supported the conclusion that when TK- was present in ganglia it was in the same neurons that contained TK+ HSV.

• Authors: R B Tenser, W A Edris
• Journal: Journal of virology (J Virol) Vol. 61 Issue 7 Pg. 2171-4 (Jul 1987) ISSN: 0022-538X [Print] United States
• PMID: 3035217 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Viral Proteins
• Thymidine Kinase

Topics

• Animals
• Female
• Genetic Complementation Test
• Keratitis, Dendritic (microbiology)
• Male
• Mice
• Simplexvirus (enzymology, genetics, physiology)
• Thymidine Kinase (genetics)
• Trigeminal Ganglion (microbiology)
• Trigeminal Nerve (microbiology)
• Viral Proteins (genetics)
• Virus Cultivation