Investigation of possible vaccine-induced epizootics of infectious bovine rhinotracheitis, using restriction endonuclease analysis of viral DNA.

Abstract

Viral DNA was extracted from each of 14 modified-live (ML) bovine herpesvirus 1 vaccines, representing all of the ML infectious bovine rhinotracheitis virus (IBRV) vaccines licensed by the US Department of Agriculture for use in cattle. Restriction endonucleases Pst I and Bgl II were used to establish restriction enzyme patterns for the vaccinal viruses. Viral DNA from isolates obtained from 6 field samples of IBRV (1 from Colorado, 1 from West Virginia, 3 from Wisconsin, 1 from South Dakota) were digested with restriction endonucleases, and patterns were compared to evaluate the role of vaccinal virus in these field epizootics of infectious bovine rhinotracheitis. Animals from which field samples were obtained had been vaccinated with ML IBRV vaccine before the epizootic of infectious bovine rhinotracheitis occurred in the herds. In 2 of the 6 field samples, DNA restriction endonuclease analyses patterns from the isolates were indistinguishable from the pattern for the vaccinal viruses used. In the remaining 4 field samples, DNA restriction endonuclease analyses patterns of the IBRV from isolates were different from those of the vaccinal viruses.

• Authors: C A Whetstone, J G Wheeler, D E Reed
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 47 Issue 8 Pg. 1789-95 (Aug 1986) ISSN: 0002-9645 [Print] United States
• PMID: 3019192 (Publication Type: Journal Article)

Chemical References

• DNA, Viral
• Viral Vaccines
• DNA Restriction Enzymes

Topics

• Animals
• Cattle
• Cell Line
• DNA Restriction Enzymes
• DNA, Viral (isolation & purification)
• Herpesvirus 1, Bovine (genetics, isolation & purification)
• Infectious Bovine Rhinotracheitis (prevention & control)
• Kidney
• Viral Vaccines