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Effect of chlordane on influenza type A virus and herpes simplex type 1 virus replication in vitro.

Abstract
The effect of chlordane on the susceptibility of Madin-Darby canine kidney cells and African Green monkey kidney cells to infection with influenza type A/PR/8/34 (HON1) virus and herpes simplex type 1 virus was determined. Exposure of both cell lines to various concentrations of chlordane for 24 h at 37 degrees C (acute exposure) effected a marked reduction in the efficiency of influenza type A virus infection, except at a dose of 0.025 ppm. Acute exposure of the monkey cells did not alter their susceptibility to herpes simplex virus infection. Viral adsorption studies at 4 and 37 degrees C revealed a marked reduction in the attachment of influenza type A virus to both cell lines following acute exposure to 10 ppm chlordane. Viral inactivation studies carried out at 4 and 37 degrees C failed to reveal differences in the level of influenza type A virus inactivation in the presence or absence of chlordane. Madin-Darby canine kidney cells exposed to 10 ppm chlordane for 60 d (chronic exposure) manifested a decrease in the efficiency of influenza type A virus infection, whereas cells chronically exposed to 0.025 ppm chlordane manifested an increase in the efficiency of influenza type A virus infection relative to mock-treated control cells. When chronically exposed cells were passaged six times in the absence of chlordane, these effects were reversed. Viral adsorption studies carried out at 4 and 37 degrees C on cells chronically exposed to 10 ppm chlordane revealed a decrease in the adsorption of influenza type A virus. Quantitation of the levels of cell-surface sialic acid, the essential terminal sugar on the receptor for influenza type A virus, indicated that the reduced adsorption of influenza type A virus to Madin-Darby canine kidney cells was not due to a loss of cell-surface sialic acid. Our findings indicate that chlordane alters the susceptibility of cells to infection with influenza type A virus but not to herpes simplex type 1 virus.
AuthorsM Beggs, J H Menna, J B Barnett
JournalJournal of toxicology and environmental health (J Toxicol Environ Health) Vol. 16 Issue 2 Pg. 173-88 ( 1985) ISSN: 0098-4108 [Print] United States
PMID3001327 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Sialic Acids
  • Chlordan
  • N-Acetylneuraminic Acid
Topics
  • Adsorption
  • Animals
  • Cell Line
  • Chlordan (pharmacology)
  • Chlorocebus aethiops
  • Dogs
  • Influenza A virus (physiology)
  • Kidney
  • N-Acetylneuraminic Acid
  • Sialic Acids (metabolism)
  • Simplexvirus (physiology)
  • Surface Properties
  • Virus Replication (drug effects)

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