Amplification and expression of phenylalanine hydroxylase in mouse erythroleukemia cells.

A cell line, CY-1, was selected in tyrosine free (tyr-) medium after fusion of mouse erythroleukemia (MEL) cells with mitomycin C-treated rat hepatoma cells. MEL cells do not express the enzyme phenylalanine hydroxylase (PH) and are unable to grow in tyr- medium, whereas the rat hepatoma cells constitutively express PH and are able to grow in tyr- medium. CY-1 cells resemble MEL cells morphologically, karyotypically, and in being inducible for hemoglobin synthesis. In contrast to MEL cells, CY-1 expresses PH and is therefore able to grow in tyr- medium. Using a rat cDNA probe for the PH gene, Southern blot analyses were carried out on DNA isolated from CY-1 and parental cells. CY-1 showed the characteristic mouse PH gene pattern but the gene copy number was amplified four- to eightfold compared to parental MEL cells.
AuthorsR M Brown, R James, R G Cotton
JournalSomatic cell and molecular genetics (Somat Cell Mol Genet) Vol. 11 Issue 3 Pg. 203-9 (May 1985) ISSN: 0740-7750 [Print] UNITED STATES
PMID2988135 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Culture Media
  • DNA, Neoplasm
  • Phenylalanine Hydroxylase
  • DNA Restriction Enzymes
  • Animals
  • Cell Fusion
  • Cell Line
  • Culture Media
  • DNA Restriction Enzymes
  • DNA, Neoplasm (genetics)
  • Electrophoresis, Agar Gel
  • Gene Amplification
  • Gene Expression Regulation
  • Leukemia, Erythroblastic, Acute (enzymology, genetics)
  • Liver Neoplasms, Experimental (enzymology, genetics)
  • Mice
  • Nucleic Acid Hybridization
  • Phenylalanine Hydroxylase (genetics)
  • Rats

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