Influence of Neurospora endonuclease on trenimon-induced structural chromosomal aberrations and sister-chromatid exchanges in human peripheral lymphocytes and Chinese hamster ovary cells.

Abstract

Human peripheral lymphocytes and Chinese hamster ovary cells were treated in the G1 phase of the cell cycle with the trifunctional alkylating agent trenimon (TRN) and post-treated with a single-strand specific endonuclease from Neurospora crassa (NE). TRN induces chromosomal aberrations of the chromatid type (CA) and sister-chromatid exchanges (SCE). NE post-treatment leads to an elevation of the frequencies of CA but not of SCEs. This indicates that TRN induced CA are the result of DNA double-strand breaks and that the SCEs originate from other types of lesions, most probably base damage.

• Authors: C Nowak, G Obe
• Journal: Mutation research (Mutat Res) Vol. 149 Issue 3 Pg. 469-74 (May 1985) ISSN: 0027-5107 [Print] Netherlands
• PMID: 2985980 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkylating Agents
• DNA, Single-Stranded
• Endonucleases
• Single-Strand Specific DNA and RNA Endonucleases
• Triaziquone

Topics

• Alkylating Agents (toxicity)
• Animals
• Cells, Cultured
• Chromosome Aberrations
• Cricetinae
• DNA, Single-Stranded (genetics)
• Endonucleases
• Female
• Humans
• Lymphocytes (drug effects)
• Single-Strand Specific DNA and RNA Endonucleases
• Sister Chromatid Exchange (drug effects)
• Triaziquone (toxicity)