Immunohistochemical distribution of S-100 protein and glial fibrillary acidic protein in normal and neoplastic salivary glands.

Abstract

Immunohistochemical localization of S-100 protein, its alpha and beta subunits, and glial fibrillary acidic protein (GFAP) in normal and neoplastic salivary glands was studied by the peroxidase-antiperoxidase method and immunoblot analysis. Positive immunostaining for S-100 protein was observed in pleomorphic adenoma, adenolymphoma, tubular adenoma, adenoid cystic carcinoma, acinic cell tumour, adenocarcinoma and carcinoma in pleomorphic adenoma. S-100 protein was localized in myoepithelial cells, epithelial cells of intercalated ducts and serous acinar cells of normal salivary gland. Both alpha and beta subunits of S-100 protein showed almost identical distribution in normal and neoplastic salivary glands, but skeletal muscle cells were alpha-positive/beta-negative whereas Schwann cells and fat cells were alpha-negative/beta-positive in the stroma and neighbouring tissue. GFAP was only found in pleomorphic adenoma and its malignant counterpart. Immunoblot analysis showed that the GFAP-related antigen consisted of several polypeptide bands with a molecular weight ranging between 35,000 to 50,000 daltons.

• Authors: Y Nakazato, Y Ishida, K Takahashi, K Suzuki
• Journal: Virchows Archiv. A, Pathological anatomy and histopathology (Virchows Arch A Pathol Anat Histopathol) Vol. 405 Issue 3 Pg. 299-310 ( 1985) ISSN: 0174-7398 [Print] Germany
• PMID: 2983489 (Publication Type: Journal Article)

Chemical References

• Antigens
• Antigens, Neoplasm
• Glial Fibrillary Acidic Protein
• Peptide Fragments
• S100 Proteins

Topics

• Adenocarcinoma (analysis)
• Adenoma, Pleomorphic (analysis)
• Antigens (analysis)
• Antigens, Neoplasm (analysis)
• Carcinoma, Adenoid Cystic (analysis)
• Epithelium (analysis)
• Glial Fibrillary Acidic Protein (analysis)
• Histocytochemistry
• Humans
• Immunoenzyme Techniques
• Parotid Neoplasms (analysis)
• Peptide Fragments (analysis)
• S100 Proteins (analysis)
• Salivary Gland Neoplasms (analysis, immunology)
• Salivary Glands (analysis, cytology, immunology)