Immunohistochemical distribution of S-100 protein and glial fibrillary acidic protein in normal and neoplastic salivary glands.

Immunohistochemical localization of S-100 protein, its alpha and beta subunits, and glial fibrillary acidic protein (GFAP) in normal and neoplastic salivary glands was studied by the peroxidase-antiperoxidase method and immunoblot analysis. Positive immunostaining for S-100 protein was observed in pleomorphic adenoma, adenolymphoma, tubular adenoma, adenoid cystic carcinoma, acinic cell tumour, adenocarcinoma and carcinoma in pleomorphic adenoma. S-100 protein was localized in myoepithelial cells, epithelial cells of intercalated ducts and serous acinar cells of normal salivary gland. Both alpha and beta subunits of S-100 protein showed almost identical distribution in normal and neoplastic salivary glands, but skeletal muscle cells were alpha-positive/beta-negative whereas Schwann cells and fat cells were alpha-negative/beta-positive in the stroma and neighbouring tissue. GFAP was only found in pleomorphic adenoma and its malignant counterpart. Immunoblot analysis showed that the GFAP-related antigen consisted of several polypeptide bands with a molecular weight ranging between 35,000 to 50,000 daltons.
AuthorsY Nakazato, Y Ishida, K Takahashi, K Suzuki
JournalVirchows Archiv. A, Pathological anatomy and histopathology (Virchows Arch A Pathol Anat Histopathol) Vol. 405 Issue 3 Pg. 299-310 ( 1985) ISSN: 0174-7398 [Print] GERMANY, WEST
PMID2983489 (Publication Type: Journal Article)
Chemical References
  • Antigens
  • Antigens, Neoplasm
  • Glial Fibrillary Acidic Protein
  • Peptide Fragments
  • S100 Proteins
  • Adenocarcinoma (analysis)
  • Adenoma, Pleomorphic (analysis)
  • Antigens (analysis)
  • Antigens, Neoplasm (analysis)
  • Carcinoma, Adenoid Cystic (analysis)
  • Epithelium (analysis)
  • Glial Fibrillary Acidic Protein (analysis)
  • Histocytochemistry
  • Humans
  • Immunoenzyme Techniques
  • Parotid Neoplasms (analysis)
  • Peptide Fragments (analysis)
  • S100 Proteins (analysis)
  • Salivary Gland Neoplasms (analysis, immunology)
  • Salivary Glands (analysis, cytology, immunology)

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