Mechanisms of immunological response induced in CD2F1 mice by administration of semisyngeneic L 1210 leukemia cells treated with cyclophosphamide.

Abstract

CD2F1 mice were immunized against semisyngeneic L 1210 leukemia. Immunization was achieved by four i.p. injections, in weekly intervals, of L 1210 cells treated in vivo twice with 200 mg/kg of cyclophosphamide. The immunized animals survived i.p. challenge with 1000 untreated L 1210 cells that was lethal for nonimmunized mice. The immunity could be abrogated in vivo with anti-mouse thymocyte serum, carrageenan or reserpine, but not by anti-mouse IgG serum, suggesting participation of T lymphocytes and macrophages in the response. Moreover, lymphocytes and macrophages from the peritoneal cavity of immunized mice were cytotoxic in vitro for L 1210 cells. The immunity, at least partially, could be adoptively transferred with peritoneal exudate cells or splenocytes.

• Authors: T Skórski, M Kawalec, J Kawiak
• Journal: Immunological investigations (Immunol Invest) Vol. 16 Issue 1 Pg. 33-43 (Mar 1987) ISSN: 0882-0139 [Print] England
• PMID: 2956188 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Reserpine
• Cyclophosphamide
• Carrageenan
• Cortisone

Topics

• Animals
• Carrageenan (pharmacology)
• Cortisone (pharmacology)
• Cyclophosphamide (pharmacology)
• Cytotoxicity, Immunologic (drug effects)
• Immunity, Cellular (drug effects)
• Immunization
• Immunization, Passive
• Leukemia L1210 (immunology)
• Macrophages (immunology)
• Mice
• Reserpine (pharmacology)
• T-Lymphocytes (immunology)
• T-Lymphocytes, Regulatory (immunology)