The detection of immune complexes of different immunoglobulin class.

Abstract

Immune complexes were detected in 51 sera from patients with a variety of immunological diseases; 14 systemic lupus erythematosus (SLE); 14 infectious mononucleosis (IM); 12 rheumatoid arthritis (RA) and 11 subacute bacterial endocarditis (SBE). Three methods were used to detect complexes: the fluid--phase Clq binding assay (Clq.BA); the solid--phaseClq binding assay (Clq.SP) and the Raji cell radio-immunoassay (RIA). Modification of the Clq.SP and the Raji cell RIA by use of monospecific antisera to immunoglobulins G, A and M enabled the class of antibody in the immune complexes to be determined. Antibodies of all three classes were found in each disease, the predominant ones being IgG and IgM in SLE and SBE, IgM and IgA in RA and IgM in IM.

• Authors: J W Quin, J A Charlesworth, G J Macdonald
• Journal: Australian and New Zealand journal of medicine (Aust N Z J Med) Vol. 9 Issue 6 Pg. 644-9 (Dec 1979) ISSN: 0004-8291 [Print] Australia
• PMID: 294921 (Publication Type: Journal Article)

Chemical References

• Antigen-Antibody Complex
• Immunoglobulin A
• Immunoglobulin G
• Immunoglobulin M
• Immunoglobulins
• Rheumatoid Factor

Topics

• Adult
• Antigen-Antibody Complex
• Arthritis, Rheumatoid (immunology)
• Centrifugation, Density Gradient
• Child
• Chromatography, Gel
• Complement Fixation Tests
• Endocarditis, Subacute Bacterial (immunology)
• Humans
• Immune System Diseases (immunology)
• Immunoglobulin A (analysis)
• Immunoglobulin G (analysis)
• Immunoglobulin M (analysis)
• Immunoglobulins (analysis)
• Infectious Mononucleosis (immunology)
• Lupus Erythematosus, Systemic (immunology)
• Middle Aged
• Molecular Weight
• Radioimmunoassay
• Rheumatoid Factor (analysis)