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Suppression of human lymphocyte proliferation by activated neutrophils or H2O2: surviving cells have an altered T helper/T suppressor ratio and an increased resistance to secondary oxidant exposure.

Abstract
Either activated neutrophils (PMNs) or nanomole amounts of reagent hydrogen peroxide (H2O2) were found to cause catalase-reversible suppression of concanavalin A (Con A)-stimulated human lymphocyte proliferation. Suppression required PMN activation and occurred with PMN/lymphocyte ratios found in peripheral blood. Inhibition by reagent H2O2 occurred with 10-40 nmol H2O2/1 X 10(6) lymphocytes, a noncytolethal amount which is produced readily by PMA-activated PMNs. Lymphoblasts recovered from suppressed cultures were two- to fourfold less sensitive than control lymphoblasts to a second exposure to H2O2. These relatively H2O2-resistant lymphoblasts also scavenge H2O2 at higher rates than do control lymphoblasts. Progeny lymphocytes from suppressed cultures contain an unusually high percentage of T helper cells with a concomitant decrease in T suppressors. These studies demonstrate a potential immunoregulatory role for phagocyte-released oxidants, especially H2O2, and suggest a possible additional mechanism to explain the excess of T helpers observed in situations of chronic inflammation.
AuthorsD C Zoschke, N D Staite
JournalClinical immunology and immunopathology (Clin Immunol Immunopathol) Vol. 42 Issue 2 Pg. 160-70 (Feb 1987) ISSN: 0090-1229 [Print] United States
PMID2948748 (Publication Type: Journal Article)
Chemical References
  • Antigens, Surface
  • Concanavalin A
  • Hydrogen Peroxide
  • Catalase
  • Tetradecanoylphorbol Acetate
Topics
  • Antigens, Surface
  • Catalase (pharmacology)
  • Cell Survival
  • Cells, Cultured
  • Concanavalin A (pharmacology)
  • Depression, Chemical
  • Drug Resistance
  • Humans
  • Hydrogen Peroxide (metabolism, pharmacology)
  • Leukocyte Count
  • Lymphocyte Activation (drug effects)
  • Neutrophils (drug effects, immunology)
  • Phenotype
  • T-Lymphocytes, Helper-Inducer (drug effects, immunology)
  • T-Lymphocytes, Regulatory (drug effects, immunology)
  • Tetradecanoylphorbol Acetate (pharmacology)

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