Abstract |
The perfusion of canine cardiac muscle with 10 microM oligomycin produced a nearly 90% slowing of the net rate of tissue ATP depletion from 0.200 to 0.025 mumol X min-1 X g wet wt-1 of tissue during a subsequent myocardial autolytic interval during which tissue pH was held constant. Moreover, lowering the tissue pH during the autolytic process by 0.6 unit from approximately 6.8 to approximately 6.2 produced a nearly 60% slowing of the net rate of tissue ATP depletion from 0.200 to 0.087 mumol X min-1 X g wet wt-1. The pH dependence of the net rate of tissue ATP depletion (by an oligomycin-sensitive process) was that predicted from the mitochondrial ATPase pH-inhibition profiles reported earlier (J. Biol. Chem. 258: 9657-9661, 1983). When taken together with our observation that the mitochondrial ATPase comprises approximately 90% of the total of all of the ATP hydrolyzing activities present in cardiac muscle cells, data reported here suggest that the protonic inhibition of the mitochondrial ATPase plays a major role in regulating the rate of tissue ATP depletion during myocardial ischemia.
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Authors | W Rouslin, J L Erickson, R J Solaro |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 250
Issue 3 Pt 2
Pg. H503-8
(Mar 1986)
ISSN: 0002-9513 [Print] United States |
PMID | 2937313
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Oligomycins
- Adenosine Triphosphate
- Adenosine Triphosphatases
- HEPES
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Topics |
- Acidosis
(metabolism)
- Adenosine Triphosphatases
(metabolism)
- Adenosine Triphosphate
(metabolism)
- Animals
- Autolysis
(enzymology, metabolism)
- Coronary Disease
(metabolism)
- Dogs
- Female
- HEPES
(pharmacology)
- Hydrogen-Ion Concentration
- Male
- Mitochondria, Heart
(enzymology)
- Myocardium
(metabolism)
- Myofibrils
(enzymology)
- Oligomycins
(pharmacology)
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