Characterization of chronic lymphocytic leukemia (CLL) cells with low density of surface membrane bound immunoglobulins (smIg).

Abstract

The study consists of 6 CLL patients with leukemic blood lymphocytes lacking T-cell characteristics and with smIg on only a very small fraction of the cells detected by microscopy or FACS analysis after direct immunofluorescence (IFL) staining. Using B-cell specific monoclonal antibodies all leukemias were found to be of the B-cell type. SmIg, mainly of IgD-class and the monotypic light chain, was detected on a large number of cells in all cases when using direct IFL. No lymphocyte clone was judged to be of the pre-B-cell type or represented fully differentiated terminal B-cells. CLL cells from the individual cases probably represent intermediate B-cell maturation steps. The results lend no support to the suggestion that this subtype of CLL necessarily belongs to an early stage of differentiation just beyond the pre-B-cell level.

• Authors: E Kimby, B Axelsson, M Björkholm, J Gordon, G Holm, H Mellstedt
• Journal: Medical oncology and tumor pharmacotherapy (Med Oncol Tumor Pharmacother) Vol. 2 Issue 4 Pg. 261-7 ( 1985) ISSN: 0736-0118 [Print] England
• PMID: 2935687 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Differentiation, T-Lymphocyte
• Antigens, Neoplasm
• Antigens, Surface
• Immunoglobulin Light Chains
• Receptors, Antigen, B-Cell
• Neprilysin
• Tetradecanoylphorbol Acetate

Topics

• Antibodies, Monoclonal
• Antigens, Differentiation, T-Lymphocyte
• Antigens, Neoplasm (analysis)
• Antigens, Surface (analysis)
• B-Lymphocytes (immunology)
• Cell Differentiation
• Flow Cytometry
• Fluorescent Antibody Technique
• Humans
• Immunoglobulin Light Chains (analysis, biosynthesis)
• Leukemia, Lymphoid (immunology)
• Neprilysin
• Receptors, Antigen, B-Cell (analysis)
• Tetradecanoylphorbol Acetate (pharmacology)