Abstract |
Thin-layer chromatography and amino acid analysis showed that urine of mutant ddY/DAO- mice lacking D-amino-acid oxidase activity contained more serine, proline, alanine and methionine than that of normal ddY/DAO+ mice. Among these four, an increase in alanine was conspicuous. However, the urinary levels of 11 other amino acids and glucose were not different between the ddY/DAO- and ddY/DAO+ mice. Amino acid analysis showed that the plasma levels of serine, proline and methionine were not elevated in the ddY/DAO- mice, though a slight increase in alanine was observed. Genetic crosses showed that aminoaciduria and lack of D-amino-acid oxidase activity were concomitantly transmitted as a set through generations. These results indicated that the lack of enzyme activity caused a specific renal aminoaciduria. Whether this enzyme merely diminishes the D- amino acid load presented for reabsorption, or actually participates catalytically in the reabsorption process, remains undetermined.
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Authors | R Konno, K Isobe, A Niwa, Y Yasumura |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 967
Issue 3
Pg. 382-90
(Dec 15 1988)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 2904279
(Publication Type: Journal Article)
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Chemical References |
- Amino Acids
- D-Amino-Acid Oxidase
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Topics |
- Amino Acids
(blood, urine)
- Animals
- Crosses, Genetic
- D-Amino-Acid Oxidase
(deficiency)
- Female
- Male
- Mice
- Mice, Inbred Strains
- Mice, Mutant Strains
- Renal Aminoacidurias
(urine)
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