Comparative studies of commercially available particle agglutination assay and enzyme-linked immunosorbent assay for screening of human T-cell leukemia virus type I antibodies in blood donors.

Abstract

Human T-cell leukemia virus type I (HTLV-I) transmission during blood transfusion can be prevented by screening for and eliminating blood containing anti-HTLV-I antibodies. For this purpose, we evaluated two commercial test kits for HTLV-I antibodies, Serodia-ATLA (particle agglutination assay [PA]) and Eitest-ATL (enzyme-linked immunosorbent assay [ELISA]), by using serum samples from Japanese blood donors. Of 2,316 serum samples, 39 (1.7%), 34 (1.5%), and 28 (1.2%) were positive for the antibody by PA, ELISA, and immunofluorescence (IF), respectively. The coincidence rate for antibody-positive and antibody-negative results was 99.5% between PA and IF, whereas it was 99.6% between ELISA and IF. The serum samples which were positive by IF were also positive by PA, whereas 2 of 26 IF-positive samples were negative by ELISA. All the samples positive by both PA and ELISA were shown to be positive by IF unequivocally. The samples positive by only one of the two methods (PA or ELISA) were different. Eleven and eight serum samples that were negative by IF but positive by PA and ELISA, respectively, were further studied for HTLV-I antibody by radioimmunoprecipitation. Three of the former but none of the latter were found to be positive for antibody. Moreover, a PA-positive but IF-negative serum sample was shown to have significantly decreased PA titers after 2-mercaptoethanol treatment. The PA was further shown to detect not only IgG antibody but also IgM antibody to HTLV-I after separation of this serum sample by high-performance liquid chromatography.

• Authors: S Kobayashi, T Yoshida, Y Hiroshige, T Matsui, N Yamamoto
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 26 Issue 2 Pg. 308-12 (Feb 1988) ISSN: 0095-1137 [Print] United States
• PMID: 2893807 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Immunoglobulin M
• Reagent Kits, Diagnostic

Topics

• Agglutination Tests
• Antibodies, Viral (analysis)
• Blood Donors
• Deltaretrovirus (immunology)
• Deltaretrovirus Infections (prevention & control)
• Enzyme-Linked Immunosorbent Assay
• Fluorescent Antibody Technique
• Humans
• Immunoglobulin M (analysis)
• Predictive Value of Tests
• Radioimmunoassay
• Reagent Kits, Diagnostic