Increased messenger RNA concentration for carbamoyl-phosphate synthase II in hepatoma 3924A.

Abstract

Previous investigations demonstrated that carbamoyl-phosphate synthase II (synthase II) (EC 6.3.5.5) activity, amount, and in vivo synthetic rate increased approximately 9-fold in the rapidly proliferating rat hepatoma 3924A compared to normal liver. This study provides evidence by Northern and RNA dot blot hybridizations of a 13-fold increase in the amount of hepatoma 3924A synthase II mRNA compared to levels in normal liver. Southern and DNA dot blots indicated amplification of CAD hepatoma 3924A synthase II gene product.

• Authors: M A Reardon, J E Dixon, G Weber
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 147 Issue 1 Pg. 494-500 (Aug 31 1987) ISSN: 0006-291X [Print] United States
• PMID: 2888462 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• CAD trifunctional enzyme
• Multienzyme Complexes
• Neoplasm Proteins
• RNA, Messenger
• RNA, Neoplasm
• Aspartate Carbamoyltransferase
• Dihydroorotase
• Ligases
• Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)

Topics

• Animals
• Aspartate Carbamoyltransferase (genetics)
• Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) (genetics)
• Cell Line
• Dihydroorotase (genetics)
• Ligases (genetics)
• Liver (physiology)
• Liver Neoplasms, Experimental (enzymology)
• Male
• Multienzyme Complexes (genetics)
• Neoplasm Proteins (genetics)
• RNA, Messenger (genetics)
• RNA, Neoplasm (genetics)
• Rats