Protection of cynomolgus monkeys against infection by human T-cell leukemia virus type-I by immunization with viral env gene products produced in Escherichia coli.

Abstract

Protection against human T-cell leukemia virus type-I (HTLV-I) infection in cynomolgus monkeys, achieved by immunizing the animals with env gene products of HTLV-I produced in Escherichia coli, was evaluated. Four monkeys that had been immunized with the env product produced antibody against HTLV-I gp68 and gp46, and their sera were found to cause strong inhibition of syncytium formation of a cat fibroblast cell line induced by HTLV-I. Immunized and non-immunized monkeys were challenged with live MT-2 cells, a high HTLV-I-producer cell line. After challenge, all the control non-immunized monkeys were infected with HTLV-I, as judged by the frequent detection of HTLV-I-antigens in cultures of their peripheral blood mononuclear cells (PBMC), whereas no antigens were recovered from PBMC of immunized monkeys. These results indicate that humoral immunity against HTLV-I-envelope protein elicited by immunization with the polypeptides synthesized in bacteria protected the monkeys against primary infection with HTLV-I.

• Authors: H Nakamura, M Hayami, Y Ohta, K Ishikawa, H Tsujimoto, T Kiyokawa, M Yoshida, A Sasagawa, S Honjo
• Journal: International journal of cancer (Int J Cancer) Vol. 40 Issue 3 Pg. 403-7 (Sep 15 1987) ISSN: 0020-7136 [Print] United States
• PMID: 2887518 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Deltaretrovirus Antibodies
• Viral Envelope Proteins
• Viral Vaccines

Topics

• Animals
• Antibodies, Viral (analysis)
• Deltaretrovirus (immunology)
• Deltaretrovirus Antibodies
• Deltaretrovirus Infections (immunology, prevention & control)
• Escherichia coli (genetics)
• Immunization
• Macaca fascicularis
• Male
• Viral Envelope Proteins (immunology)
• Viral Vaccines (immunology)