Incidence of experimental fascioliasis on the activity of drug-metabolizing enzymes in lamb liver.

Abstract

The purpose of this investigation was to determine the effects of a subclinical fascioliasis at various stages of its development (by week--4, 8, 12, and 16 weeks after the infestation by an oral administration of 150 metacercariae of Fasciola hepatica) on the activity of some hepatic drug-metabolizing systems in lamb. The parasitic pathology was ascertained at autopsy and by clinical observation of animals. Hepatic microsomal cytochrome P-450 content was significantly decreased (by 9-22%) in all infected groups of animals. In early stages of the parasitic disease, decreases in cytochrome b5 content (10-18%) and ethoxycoumarin O-deethylase (25%) were observed, whereas aminopyrine N-demethylase, benzphetamine N-demethylase, and aniline hydroxylase were significantly lowered by 8 to 16 weeks postinfection. Among investigated transferases, glutathione transferase was only decreased (28%) in animals killed 16 weeks after the infestation; in these animals a significant increase in microsomal gamma-glutamyltransferase was observed, probably related to the elevated plasma activity of this enzyme. By 8 weeks postinfection, a simultaneous increase in cytosolic calcium (38%) and decrease in cytosolic glutathione (22%) would correspond to an oxidative cell injury occurring in the course of fascioliasis. The consequences of the fascioliasis-induced decreases in liver-oxidative and conjugative liver drug metabolism are discussed.

• Authors: P Galtier, G Larrieu, A E Tufenkji, M Franc
• Journal: Drug metabolism and disposition: the biological fate of chemicals (Drug Metab Dispos) 1986 Jan-Feb Vol. 14 Issue 1 Pg. 137-41 ISSN: 0090-9556 [Print] United States
• PMID: 2868858 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Pharmaceutical Preparations
• Cytochrome P-450 Enzyme System
• L-Iditol 2-Dehydrogenase
• Oxygenases
• 7-Alkoxycoumarin O-Dealkylase
• Glutamate Dehydrogenase
• gamma-Glutamyltransferase

Topics

• 7-Alkoxycoumarin O-Dealkylase
• Animals
• Cytochrome P-450 Enzyme System (analysis)
• Fascioliasis (enzymology)
• Glutamate Dehydrogenase (analysis)
• L-Iditol 2-Dehydrogenase (analysis)
• Liver (enzymology)
• Male
• Oxygenases (analysis)
• Pharmaceutical Preparations (metabolism)
• Sheep
• gamma-Glutamyltransferase (analysis)