Abstract |
The production of fluorescent end-products with ampicillin as substrate can be used to detect beta-lactamase activity in biological fluids. A fluorescent spot test was evaluated as a rapid method of detecting beta-lactamase in the urethral exudates of men infected with Neisseria gonorrhoeae in Thailand. Among 208 men with culture-proven gonococcal urethritis, the fluorescent spot test was positive in 92 of 101 men from whom penicillinase-producing N gonorrhoeae (PPNG) were isolated and in 4 of 107 men from whom non-PPNG were isolated. The fluorescent spot test is a rapid, inexpensive, and sensitive method to detect PPNG and may be valuable in determining appropriate treatment for patients with gonococcal urethritis.
|
Authors | D N Taylor, K C Chen, K Panikabutra, C Wongba, A Chitwarakern, P Echeverria, K K Holmes |
Journal | Lancet (London, England)
(Lancet)
Vol. 2
Issue 8456
Pg. 625-6
(Sep 21 1985)
ISSN: 0140-6736 [Print] England |
PMID | 2863630
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Penicillinase
- beta-Lactamases
|
Topics |
- Exudates and Transudates
(microbiology)
- Fluorescence
- Gonorrhea
(microbiology)
- Humans
- Male
- Methods
- Neisseria gonorrhoeae
(enzymology, isolation & purification)
- Penicillinase
(biosynthesis)
- Urethra
(microbiology)
- beta-Lactamases
(analysis)
|