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Expression of the Epstein-Barr virus encoded EBNA-1 gene in stably transfected human and murine cell lines.

Abstract
Five murine and 3 human tumor cell lines were transfected with a retroviral vector that carries the EBV encoded EBNA-1 gene. All cell lines expressed intranuclear EBNA-1 as detected by anticomplement immunofluorescence and Western blot assays. The cell lines differed in the level of EBNA-1 expression and the size of the protein. The internal major late promoter of adenovirus was efficient in directing the transcription of EBNA-1 in the human lymphoma line BJAB, the murine T-cell lymphoma Tikaut, RBL-5, EL-4 and in the mouse sarcoma line MSWBS but was less efficient in Ramos, an EBV negative Burkitt lymphoma line, the human T-cell leukemia line 1301TK and the P815-X2 mouse mastocytoma line. All transfected lines except MSWBS contained EBNA-1 in a truncated form. The truncated EBNA-1 polypeptide reacted with the conventional human antibody reagents in an EBNA specific fashion but failed to bind rabbit or human antibody directed against the glycine-alanine repeat sequence. MSWBS contained a truncated as well as a full size EBNA-1 polypeptide. It also reacted with antibody directed against the glycine-alanine repeat. This indicates that the repeat sequence is regularly affected by the truncation.
AuthorsG V Patel, M G Masucci, G Winberg, G Klein
JournalInternational journal of cancer (Int J Cancer) Vol. 42 Issue 4 Pg. 592-8 (Oct 15 1988) ISSN: 0020-7136 [Print] United States
PMID2844682 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Viral
  • Epstein-Barr Virus Nuclear Antigens
Topics
  • Adenoviridae (genetics)
  • Animals
  • Antigens, Viral (analysis, genetics)
  • Cell Line
  • Epstein-Barr Virus Nuclear Antigens
  • Gene Rearrangement
  • Herpesvirus 4, Human (isolation & purification)
  • Humans
  • Mice
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Transfection

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