Analysis of the TNF receptor on KYM cells by binding assay and affinity cross-linking.

Abstract

The existence of a tumor necrosis factor (TNF) receptor on KYM human myosarcoma cells was elucidated by Scatchard plot and cross-linking analysis. The average number of specific binding sites per cell was 15,300 and the apparent dissociation constant Kd was 4.0 X 10(-10) M. The estimated relative molecular mass of a TNF receptor on KYM cell was approximately 95,000.

• Authors: Y Niitsu, N Watanabe, H Sone, H Neda, N Yamauchi, M Maeda, I Urushizaki
• Journal: Journal of biological response modifiers (J Biol Response Mod) Vol. 7 Issue 3 Pg. 276-82 (Jun 1988) ISSN: 0732-6580 [Print] United States
• PMID: 2839624 (Publication Type: Journal Article)

Chemical References

• Receptors, Cell Surface
• Receptors, Tumor Necrosis Factor

Topics

• Cell Line
• Electrophoresis, Polyacrylamide Gel
• Humans
• Kinetics
• Molecular Weight
• Myosarcoma (analysis)
• Receptors, Cell Surface (analysis)
• Receptors, Tumor Necrosis Factor
• Sarcoma (analysis)