A spin-label study of membrane proteins and internal microviscosity of erythrocytes in hereditary spherocytosis.

Abstract

Electron spin resonance (ESR) spectra of erythrocyte membranes of patients with hereditary spherocytosis (HS) and of healthy controls labeled with a maleimide spin label did not differ significantly both before and after prolonged incubation at 37 degrees C. It suggests that the different behavior of spin-labeled HS erythrocyte membranes upon incubation at a higher temperature reported previously is due indeed to structural abnormalities of HS red cell membranes and not to alterations in their proteolytic activity. Measurements of the rotational correlation time of Tempamine spin probe demonstrated a significant elevation of internal microviscosity of erythrocytes in HS, more pronounced in non-splenectomized patients.

• Authors: G Bartosz, M Gaczyńska, E Grzelińska, L Judkiewicz
• Journal: Life sciences (Life Sci) Vol. 41 Issue 20 Pg. 2285-8 (Nov 16 1987) ISSN: 0024-3205 [Print] Netherlands
• PMID: 2824948 (Publication Type: Journal Article)

Chemical References

• Cyclic N-Oxides
• Membrane Proteins
• Spin Labels
• 4-amino-TEMPO

Topics

• Adolescent
• Adult
• Child
• Child, Preschool
• Cyclic N-Oxides (metabolism)
• Electron Spin Resonance Spectroscopy
• Erythrocyte Membrane (analysis)
• Humans
• Membrane Proteins
• Spherocytosis, Hereditary (blood)
• Spin Labels (metabolism)
• Splenectomy
• Viscosity