Induction of the RI cAMP-binding protein in the "neurite-minus" N1A-103 mouse neuroblastoma cell line.

Abstract

We examined the effects of agents/conditions that raise intracellular cAMP concentration in the induction of the 47,000-dalton RI cAMP-binding protein in the "neurite-minus" N1A-103 mouse neuroblastoma cells. The amount of RI in cell extracts was quantitated by photoaffinity labeling with 8-N3-[32P]cAMP and by ELISA and Western blot. Our results showed that treatment of the N1A-103 neuroblastoma cells with 20 microM forskolin or 1 mM dibutyryl cAMP, or reducing the serum concentration in the culture medium from 10% to 1% caused a 3-4 fold increase in the amount of RJ. Our results suggest that the induction of RJ can occur independent of morphological differentiation in the mouse neuroblastoma cells.

• Authors: A Y Liu, V Kamalakannan, Z Lin
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 148 Issue 1 Pg. 350-6 (Oct 14 1987) ISSN: 0006-291X [Print] United States
• PMID: 2823808 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Affinity Labels
• Azides
• Carrier Proteins
• Cyclic AMP Receptor Protein
• 8-azidoadenosine-3',5'-monophosphate
• Cyclic AMP

Topics

• Affinity Labels (metabolism)
• Animals
• Azides (metabolism)
• Carrier Proteins (analysis, biosynthesis, metabolism)
• Cell Line
• Cyclic AMP (analogs & derivatives, metabolism)
• Cyclic AMP Receptor Protein
• Cytosol (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Kinetics
• Mice
• Neuroblastoma (metabolism)