[Hybridomas producing monoclonal antibodies to Crimean hemorrhagic fever virus].

Abstract

A series of hybridomas producing monoclonal antibodies to Crimean hemorrhagic fever virus was obtained and their cultural properties were characterized. HEMA-12 and HEMA-24 secreted monoclonal IgG2b antibodies, HEMA-101 secreted monoclonal IgG1 antibodies, HEMA-31, HEMA-9 and HEMA-11 secreted monoclonal IgG2 antibodies. According to the results of the indirect immunofluorescence test, the titer of specific immunoglobulins in the culture fluid was 1:16-1:32, but sometimes reached 1:64-1:128. The titer of antibodies in ascitic fluid amounted to dilutions of 10(4)-10(5). Hybridomas were cloned by the method of ultimate dilutions. The injection of 5-15 million HEMA cells into the abdominal cavity of BALB/c mice induced the formation of ascitic tumors in the animals within 7-11 days and the accumulation of ascitic fluid in a volume of 1-4 ml. Hybridomas, found to be capable of passage from mouse to mouse, underwent 5-16 passages during the term of observation.

• Authors: T M Shutkova, E E Mel'nikova, S Ia Gaĭdamovich, A S Novokharskiĭ, A L Turchinskaia
• Journal: Zhurnal mikrobiologii, epidemiologii i immunobiologii (Zh Mikrobiol Epidemiol Immunobiol) Issue 6 Pg. 102-7 (Jun 1989) ISSN: 0372-9311 [Print] Russia (Federation)
• Vernacular Title: Gibridomy, produtsiruiushchie monoklonal'nye antitela k virusu Krymskoĭ gemorrhagicheskoĭ likhoradki.
• PMID: 2800788 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Immunoglobulins

Topics

• Animals
• Antibodies, Monoclonal (biosynthesis)
• Hemorrhagic Fever, Crimean (immunology)
• Hybridomas (immunology)
• Immunoglobulins (biosynthesis)
• Mice
• Mice, Inbred BALB C