Abstract |
We have defined some characteristics of the mouse Mx protein as a marker of biological response to interferon (IFN) and to virus infection in A2G mice. The Mx protein has been detected and quantitated by Western immunoblot analysis. Upon induction by poly(I): poly(C) or with Newcastle disease virus, the Mx protein is expressed and accumulated in a variety of organs, such as liver, lungs, spleen, kidneys, heart, and brain. In some organs the expression of the Mx protein is detected readily, as soon as 4 h after treatment. The highest protein level is reached at 24 h, and it remains stable for several days declining slowly to return to preinduced levels 2-3 weeks after treatment. Infection with an hepatotropic or a pneumotropic strain of influenza virus resulted in a systemic induction of Mx protein, the highest levels being found in the target organ for virus replication. Our results indicate that the Mx protein is a sensitive, quantitative, and stable marker to follow IFN activity or virus infection in an animal model.
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Authors | M A Horisberger, K De Staritzky |
Journal | Journal of interferon research
(J Interferon Res)
Vol. 9
Issue 5
Pg. 583-90
(Oct 1989)
ISSN: 0197-8357 [Print] United States |
PMID | 2794581
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Interferon Inducers
- Mx1 protein, mouse
- Myxovirus Resistance Proteins
- GTP-Binding Proteins
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Topics |
- Animals
- Biomarkers
- GTP-Binding Proteins
- Influenza A virus
(immunology)
- Interferon Inducers
(pharmacology)
- Mice
- Myxovirus Resistance Proteins
- Newcastle disease virus
(immunology)
- Protein Biosynthesis
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