Eleven invasive and five non-invasive primary vaginal
carcinomas were studied by
DNA flow cytometry using archival
paraffin-embedded tissue as starting material. Overall frequency of
DNA aneuploidy in the invasive
carcinomas was 8/11 (73%).
DNA aneuploidy occurred in all four advanced stage (III-IV) and in 4/7 (57%) of the early stage (I-II)
carcinomas. Among the
squamous cell carcinomas aneuploid DNA content was also associated with non-keratinizing
tumor type. Invasive vaginal
carcinomas showed a high median S-phase fraction (SPF) (18.4%, range 6.9-31.8%). High SPF values were associated with advanced stage and non-keratinizing
tumors. Corrected 5-year survival rate in invasive
vaginal cancer was 44%, with no significant relation to
DNA ploidy or SPF. In situ
carcinomas were almost as often
DNA-
aneuploid (3/5, 60%) as the invasive
carcinomas and had comparable median SPF value (13.4%, range 5.5-24.6). One in situ
carcinoma with a high
DNA-index and SPF relapsed, but overall 5-year survival rate was 100%. In conclusion, both invasive and in situ vaginal
carcinomas frequently contain
DNA-
aneuploid stemlines and show a high SPF. Although
DNA aneuploidy and high SPF correlate with advanced stage and non-keratinizing
tumor type, they do not have much prognostic relevance in vaginal
neoplasia.