Abstract |
The antigenic profile and the proliferative pathways in leukemic cells from the patient TRT with T- prolymphocytic leukemia (T-PLL) were analyzed using monoclonal antibodies (MoAbs) and cytokines. T-PLL cells expressed the phenotype CD1- CD3+ CD4+ CD8+. Incubation with the differentiating agent phorbol-12-myristate-13-acetate markedly increased the percentage of cells with the CD4- CD8+ phenotype, suggesting that leukemic cells were already committed towards a differentiated element with the CD4- CD8+ phenotype. T-PLL cells were induced to proliferate by anti-CD2 MoAb 9-1 + 9.6 and by anti-CD3 MoAb OKT3. The two pathways exhibited normal functional interactions and were susceptible to modulation by anti-HLA class I MoAbs. These results indicate that regulation of cell proliferation was preserved to a significant extent in the T-PLL cells analyzed. At variance with normal resting T cells that require previous activation to proliferate when incubated with interleukin-1 (IL-1) or interleukin-2 (IL-2), T-PLL cells proliferated vigorously when incubated with either interleukin. Furthermore, T-PLL cells proliferated when incubated with immune interferon (IFN-gamma). The latter finding parallels the enhancement by IFN-gamma of the proliferative response of lectin-activated murine T lymphocytes. These results suggest that T-PLL cells, which express a high constitutive level of c-myc mRNA, may be in an activated state. The antigenic phenotype and the characteristics of the proliferative pathways of T-PLL cells from the patient TRT are compatible with the possibility that they may be derived from an intermediate thymocyte.
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Authors | M C Turco, M De Felice, F Alfinito, A Lamberti, F Costanzo, M Giordano, V Martinelli, B Rotoli, S Ferrone, S Venuta |
Journal | Blood
(Blood)
Vol. 74
Issue 5
Pg. 1651-7
(Oct 1989)
ISSN: 0006-4971 [Print] United States |
PMID | 2790191
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, CD
- Interleukin-1
- Interleukin-2
- Interleukin-6
- Recombinant Proteins
- Tetradecanoylphorbol Acetate
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Topics |
- Antibodies, Monoclonal
- Antigens, CD
(analysis)
- Cell Differentiation
- Cells, Cultured
- Humans
- Interleukin-1
(pharmacology)
- Interleukin-2
(pharmacology)
- Interleukin-6
(pharmacology)
- Kinetics
- Leukemia, Prolymphocytic
(immunology)
- Lymphocyte Activation
(drug effects)
- Microscopy, Electron
- Recombinant Proteins
(pharmacology)
- Reference Values
- T-Lymphocytes
(drug effects, immunology, ultrastructure)
- Tetradecanoylphorbol Acetate
(pharmacology)
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