Binding of [3H]inositoltrisphosphate and [3H]phorbol 12,13-dibutyrate in rat hippocampus following transient global ischemia: a quantitative autoradiographic study.

Abstract

An in vitro quantitative autoradiographic binding study of the phosphatidylinositol system ligands [3H]inositol 1,4,5-trisphosphate (IP3) and [3H]phorbol 12,13-dibutyrate (PDBU) to rat brain slices was performed at 6, 12, 24, 28 and 72 h following a 20 min ischemic injury. PDBU binding showed a transient 20% decrease in the dentate gyrus and the CA3 the first 24 h as well as a 50% decrease in the CA1 at 72 h. A 50% decline in IP3 binding was seen in all regions at 6-12 h except the pyramidal cell layer of the CA1. This downregulation of calcium mobilizing intracellular receptors is probably a defence against ischemic neuronal cell death.

• Authors: M B Jørgensen, J Deckert, D C Wright
• Journal: Neuroscience letters (Neurosci Lett) Vol. 103 Issue 2 Pg. 219-24 (Aug 28 1989) ISSN: 0304-3940 [Print] Ireland
• PMID: 2788841 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Inositol Phosphates
• Sugar Phosphates
• Phorbol 12,13-Dibutyrate
• Inositol 1,4,5-Trisphosphate
• Calcium

Topics

• Animals
• Autoradiography
• Calcium (metabolism)
• Hippocampus (metabolism, pathology)
• Inositol 1,4,5-Trisphosphate
• Inositol Phosphates (metabolism)
• Ischemic Attack, Transient (metabolism)
• Male
• Phorbol 12,13-Dibutyrate (metabolism)
• Rats
• Rats, Inbred Strains
• Sugar Phosphates (metabolism)
• Time Factors