Tumour necrosis factor production by IL-2-activated macrophages in vitro and in vivo.

Abstract

Purified human and murine monocytes can be activated in vitro by human recombinant IL-2 to secrete tumour necrosis factor (TNF). TNF mRNA is detected within 4 h of addition of IL-2. Signal transduction does not appear to require expression of the Tac peptide of the IL-2 receptor. IL-2 does, however, induce Tac mRNA and Tac peptide expression on peripheral blood monocytes (PBM). In addition to these in vitro studies, PBM retrieved from IL-2-treated cancer patients and mice have been shown to have a greatly increased capacity for TNF production. These studies identify a pathway for macrophage activation by IL-2 both in vitro and in vivo. They also suggest a mechanism for some of the immunoregulatory and toxic effects of IL-2 based immunotherapy regimens.

• Authors: J S Economou, W H McBride, R Essner, K Rhoades, S Golub, E C Holmes, D L Morton
• Journal: Immunology (Immunology) Vol. 67 Issue 4 Pg. 514-9 (Aug 1989) ISSN: 0019-2805 [Print] England
• PMID: 2788610 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Interleukin-2
• Recombinant Proteins
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Cells, Cultured
• Humans
• Interleukin-2 (pharmacology)
• Macrophage Activation
• Macrophages (drug effects, metabolism)
• Mice
• Mice, Inbred C3H
• Recombinant Proteins (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)