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Human insulin receptor radioimmunoassay: applicability to insulin-resistant states.

Abstract
A radioimmunoassay of the human insulin receptor was developed employing a potent rabbit polyclonal antibody to the human insulin receptor and a highly purified human placental insulin receptor preparation. The receptor, obtained by sequential affinity chromatography with insulin receptor monoclonal antibody-agarose and wheat germ agglutinin-agarose, was radiolabeled with 125I-Bolton-Hunter reagent at specific activities of 2,100-3,300 Ci/mmol. Over 75% of this ligand was immunoprecipitable with the polyclonal antireceptor antibody and remained immunoprecipitable for greater than 45 days. The assay was sensitive to unlabeled receptor concentrations as low as 0.2 ng/0.5 ml; unlabeled insulin did not cross-react and unlabeled insulin-like growth factor (IGF)-I receptor cross-reacted weakly. The radioimmunoassay was applicable to the measurement of insulin receptors in tissues and cells that were extracted by solubilization in 1% Triton X-100; no purification of the extracted receptor was necessary. Of the three major target tissues for insulin action studied, liver had the highest concentration of receptors (47.6 ng/mg protein); fat and muscle had lower levels. Other studies with the radioimmunoassay indicated that insulin receptors were decreased both in monocytes from obese hyperinsulinemic subjects and in fibroblasts from patients with leprechaunism.
AuthorsV Pezzino, V Papa, V Trischitta, A Brunetti, P A Goodman, M K Treutelaar, J A Williams, B A Maddux, R Vigneri, I D Goldfine
JournalThe American journal of physiology (Am J Physiol) Vol. 257 Issue 3 Pt 1 Pg. E451-7 (Sep 1989) ISSN: 0002-9513 [Print] United States
PMID2782405 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Receptor, Insulin
Topics
  • Female
  • Humans
  • Insulin Resistance
  • Placenta (analysis, ultrastructure)
  • Pregnancy
  • Radioimmunoassay (methods)
  • Receptor, Insulin (analysis)

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