Antibodies against allantoic fluid of chick embryos detected in Japanese sera.

Abstract

Japanese sera were shown by enzyme-linked immunosorbent assay (ELISA) to contain frequently antibodies against allantoic fluid, confirming the results obtained in our previous study on ELISA for the detection of mumps antibodies. Chromatographic analysis with Sephacryl S-300 of the allantoic fluid antigen used for ELISA suggested the antigens for these antibodies in Japanese sera to be macromolecular proteins. Comparative absorption tests with allantoic fluid and ovomacroglobulin were therefore carried out on serum samples positive in ELISA with allantoic fluid antigen, and ovomacroglobulin was identified to be the antigenic component for antibodies to allantoic fluid antigen in Japanese sera.

• Authors: H Kawashima, K Sasaki, M Nakagawa, T Mori, S Makino, M Matumoto
• Journal: The Kitasato archives of experimental medicine (Kitasato Arch Exp Med) Vol. 62 Issue 1 Pg. 39-44 (Apr 1989) ISSN: 0023-1924 [Print] Japan
• PMID: 2770127 (Publication Type: Journal Article)

Chemical References

• Antibodies

Topics

• Allantois (immunology)
• Animals
• Antibodies (analysis)
• Chick Embryo
• Enzyme-Linked Immunosorbent Assay
• Extraembryonic Membranes (immunology)
• Fetal Blood (immunology)
• Humans