HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

In situ hybridization for procollagen types I, III and IV mRNA in normal and fibrotic rat liver: evidence for predominant expression in nonparenchymal liver cells.

Abstract
The expression of alpha 2(I), alpha 1(III) and alpha 1(IV) procollagen mRNA was analyzed in normal and CCl4-induced fibrotic rat liver by in situ hybridization using RNA probes. In normal liver, moderate amounts of alpha 2(I) and alpha 1(III) procollagen transcripts were found in sinusoidal cells, in stromal cells of the portal tracts and in the vicinity of central veins, whereas a1(IV) procollagen gene expression was below the threshold of detection. After 2 weeks of CCl4 treatment, increased transcription of alpha 2(I) and alpha 1(III) procollagen genes was observed in sinusoidal cells. At this stage, alpha 1(IV) procollagen mRNA was detectable in the same cell types and localization as alpha 2(I) and alpha 1(III) procollagen transcripts, although with a weaker signal. After 4 weeks, newly formed fibrous septa showed many cells intensely labeled by alpha 2(I), alpha 1(III) and alpha 1(IV) procollagen probes. Neither in normal liver nor at any stage of fibrosis was any hybridization signal above background observed in hepatocytes. These patterns suggest that in the liver Type I, Type III and Type IV procollagen expression takes place predominantly in nonparenchymal cells. Therefore, hepatocytes do not appear to be significantly involved in procollagen production in this experimental model of liver fibrosis.
AuthorsS Milani, H Herbst, D Schuppan, E G Hahn, H Stein
JournalHepatology (Baltimore, Md.) (Hepatology) Vol. 10 Issue 1 Pg. 84-92 (Jul 1989) ISSN: 0270-9139 [Print] United States
PMID2737606 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Procollagen
  • RNA Probes
  • RNA, Messenger
  • Carbon Tetrachloride
Topics
  • Animals
  • Blotting, Northern
  • Carbon Tetrachloride
  • Female
  • Liver (metabolism, pathology)
  • Liver Cirrhosis, Experimental (chemically induced, metabolism, pathology)
  • Nucleic Acid Hybridization
  • Procollagen (genetics)
  • RNA Probes
  • RNA, Messenger (metabolism)
  • Rats
  • Rats, Inbred Strains
  • Reference Values
  • Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: