Abstract |
The role of IgE antibodies against respiratory syncytial virus has attracted attention for both human and bovine disease. To detect such antibodies, we have developed an enzyme-linked immunosorbent assay (ELISA) specific for bovine respiratory syncytial virus (BRSV). Firstly, antiserum strongly positive for BRSV-specific IgE was produced by immunizing a levamisole-treated calf with BRSV. The presence and specificity of BRSV-specific IgE in this animal was confirmed with the Praunitz-Kustner (PK) technique. Potential interference in an ELISA by other BRSV-specific immunoglobulin isotypes was eliminated by preferential precipitation of serum samples with 27.5% saturated ammonium sulfate. The correlation between the PK and the assay was greater than 93% and the ELISA was found to be more specific than the PK. Indeed, in a pilot experimental infection study, the serum levels of BRSV-specific IgE were found to correlate with the symptom expression following repetitive live virus aerosolization. This may prove to be a useful rapid test to study both herd immunity and the potential pathogenic influence of IgE.
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Authors | R S Stewart, L J Gershwin |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 20
Issue 4
Pg. 313-23
(Mar 1989)
ISSN: 0165-2427 [Print] Netherlands |
PMID | 2718372
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antibodies, Viral
- Immune Sera
- Immunoglobulin E
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Topics |
- Animals
- Antibodies, Viral
(analysis)
- Antibody Specificity
- Cattle
- Cattle Diseases
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Immune Sera
(immunology)
- Immunoglobulin E
(analysis)
- Predictive Value of Tests
- Respiratory Syncytial Viruses
(immunology)
- Respirovirus Infections
(immunology, veterinary)
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