Neoplastic transformation of human keratinocytes by polybrene-induced DNA-mediated transfer of an activated oncogene.

Polybrene, in conjunction with dimethyl sulfoxide (DMSO) shock has been shown to increase the frequency of DNA-mediated gene transfer to mammalian cells as compared with the frequency obtained with calcium phosphate transfection. We have successfully adapted this procedure for use with human epidermal keratinocytes. Non-tumorigenic human epidermal epithelial cells immortalized by SV40 tumor antigen were neoplastically transfected, using Polybrene at a concentration of 10 micrograms ml-1, followed by a 4 min shock, with 30% DMSO, with a plasmid carrying the activated H-ras gene from the EJ bladder carcinoma cell line. The transfected cells showed morphological alterations and induced carcinomas when transplanted into nude mice. They contained integrated copies of the transfected H-ras gene and expressed high levels of the p21 protein. Polybrene-induced DNA transfection, therefore, offers the opportunity to transfer genes effectively into human epidermal keratinocytes and should accelerate the study of the interaction between oncogenes and human epithelial cells. This study appears to represent the first neoplastic conversion of nontumorigenic, immortalized human epidermal keratinocytes by an activated human oncogene.
AuthorsJ S Rhim, J B Park, G Jay
JournalOncogene (Oncogene) Vol. 4 Issue 11 Pg. 1403-9 (Nov 1989) ISSN: 0950-9232 [Print] ENGLAND
PMID2682464 (Publication Type: Journal Article)
Chemical References
  • Polyamines
  • Hexadimethrine Bromide
  • Dimethyl Sulfoxide
  • Animals
  • Cell Line
  • Cell Transformation, Neoplastic
  • Dimethyl Sulfoxide (pharmacology)
  • Genes, ras
  • Hexadimethrine Bromide (pharmacology)
  • Humans
  • Karyotyping
  • Keratinocytes (cytology, drug effects)
  • Kinetics
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Plasmids
  • Polyamines (pharmacology)
  • Transfection (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: