Abstract | PURPOSE: Testicular sperm extraction (TESE) for intracytoplasmic sperm injection (ICSI) was first introduced for the treatment of non-obstructive azoospermia. This study was conducted to detect predictive factors affecting the success of microTESE. MATERIALS AND METHODS: We retrospectively evaluated the results of 191 cases who underwent microTESE. For each patient, the testicular volume, endocrine profile [ follicle stimulating hormone (FSH), luteinizing hormone (LH), free testosterone (FT), total testosterone (TT)], serum inhibin B level, karyotype analysis, and Y chromosome microdeletions were recorded, and all data were analyzed to detect any predictors. The receiver operating characteristic curve, two-sample t-test and regression analysis were used for the statistical analysis. RESULTS: The mean age of the patients was 34.4 ± 5.6 years. Sperm retrieval was successful in 104 (54.5%) patients, and there was no sperm in 87 (45.5%). Seven factors including, testicular size, Johnson score, Y chromosome microdeletion, and serum FSH, LH, FT and TT levels were different between the successful and unsuccessful groups. Six patients had Klinefelter syndrome, and ten patients (5.2%) had a Y chromosome microdeletion (5 AZF-c, 1 AZF- b, 2 AZF-bc, 1 AZF-abc, and 1 AZF-ac). The Johnson score, TT level, family history and Y chromosome microdeletions were determined to be independent predictive factors for sperm found. According to the testicular histology, the sperm-found ratios were 36%, 48.6%, and 95.5% in the sertoli cell only syndrome, maturation arrest, and hypospermatogenesis groups, respectively. CONCLUSION: According to our results, the Johnson score, TT level, family history-related infertility, and Y chromosome microdeletions were determined to be independent predictive factors for sperm found.
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Authors | Mehmet Cetinkaya, Kadir Onem, Orhan Unal Zorba, Hamdi Ozkara, Bulent Alici |
Journal | Urology journal
(Urol J)
Vol. 12
Issue 6
Pg. 2436-43
(Dec 23 2015)
ISSN: 1735-546X [Electronic] Iran |
PMID | 26706742
(Publication Type: Journal Article)
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Chemical References |
- inhibin B
- Testosterone
- Inhibins
- Luteinizing Hormone
- Follicle Stimulating Hormone
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Topics |
- Adult
- Azoospermia
(blood, etiology, surgery)
- Chromosome Deletion
- Chromosomes, Human, Y
- Dissection
- Follicle Stimulating Hormone
(blood)
- Humans
- Infertility, Male
- Inhibins
(blood)
- Karyotype
- Klinefelter Syndrome
(complications)
- Luteinizing Hormone
(blood)
- Male
- Microsurgery
- Organ Size
- Predictive Value of Tests
- Retrospective Studies
- Sex Chromosome Aberrations
- Sex Chromosome Disorders of Sex Development
(complications)
- Sperm Retrieval
- Testis
(pathology)
- Testosterone
(blood)
- Treatment Outcome
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