Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo.

Abstract

Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg-1, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.

• Authors: Mircea Dumitru Croitoru, Hermina Iulia Petkes, Ibolya Fülöp, Remus Cotârlan, Oana Elena Şerban, Titica Maria Dogaru, Şerban Andrei Gâz Florea, Béla Tőkés, Cornelia Majdik
• Journal: Acta pharmaceutica (Zagreb, Croatia) (Acta Pharm) Vol. 65 Issue 4 Pg. 413-26 (Dec 2015) ISSN: 1846-9558 [Electronic] Poland
• PMID: 26677898 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Nitrates
• Nitric Oxide Donors
• Nitrites
• Nitrogen Oxides
• Vasodilator Agents
• nitrones
• Nitric Oxide
• Hydroxyl Radical

Topics

• Animals
• Biomarkers (blood)
• Hydroxyl Radical (chemistry)
• Injections, Intraperitoneal
• Injections, Intravenous
• Liver (metabolism)
• Molecular Structure
• Nitrates (blood)
• Nitric Oxide (blood, chemistry, metabolism)
• Nitric Oxide Donors (administration & dosage, blood, chemistry, metabolism)
• Nitrites (blood)
• Nitrogen Oxides (administration & dosage, blood, chemistry, metabolism)
• Rabbits
• Spin Trapping
• Time Factors
• Vasodilator Agents (administration & dosage, blood, chemistry, metabolism)