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Effect of ionomycin on interaction of calnexin with vesicular stomatitis virus glycoprotein is cell type-specific.

Abstract
Ionomycin is a calcium ionophore that induces release of calcium ions (Ca(2+)) from cellular storage to cytoplasm and Ca(2+) influx from the outside of the cell. We investigated the effect of ionomycin on endoplasmic reticulum (ER)-Golgi transport in the vesicular stomatitis virus glycoprotein (VSV-G) system. Ionomycin inhibited transport of VSV-G in a concentration-dependent manner in baby hamster kidney (BHK) cells and HeLa cells. Half-maximum inhibition was observed at 5 μM. The inhibitory effect of ionomycin was not dependent on the cytoplasmic portion. Chelation of Ca(2+) in culture medium did not affect transport efficiency, but co-incubation with ionomycin completely shut off transport. These findings highlight the importance of Ca(2+) release from cellular storage. Because the inhibitory effect of ionomycin was expected to be dependent on mutual interaction of VSV-G and the ER chaperone calnexin, we further investigated interaction kinetics. In HeLa cells but not BHK cells the interaction of VSV-G and calnexin was prolonged in the presence of ionomycin. Taken together, the present results indicate that, by releasing Ca(2+) from cellular storage, ionomycin inhibits ER-Golgi transport by interfering with the release of VSV-G from calnexin in HeLa cells. A mechanism of cell type-dependent ER-Golgi transport regulation was revealed.
AuthorsMichisato Okudera, Takahiro Gojoubori, Ichiro Tsujino, Masatake Asano
JournalJournal of oral science (J Oral Sci) Vol. 57 Issue 4 Pg. 305-12 ( 2015) ISSN: 1880-4926 [Electronic] Japan
PMID26666853 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Glycoproteins
  • Viral Proteins
  • Calnexin
  • Ionomycin
Topics
  • Animals
  • Calnexin (metabolism)
  • Cell Line
  • Cricetinae
  • Endoplasmic Reticulum (metabolism)
  • Glycoproteins (metabolism)
  • Golgi Apparatus (metabolism)
  • HeLa Cells
  • Humans
  • Ionomycin (pharmacology)
  • Vesicular Stomatitis (metabolism)
  • Viral Proteins (metabolism)

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