The experiment is designed to explore pathological festures and material basis of pseadoanaphylactoid reaction induced by notoginseng total
saponin preparation. Mouse pseadoanaphylactoid reaction was used, 50 ICR mice were randomly assigned to control group, positive medicine group, notoginseng total
saponin preparation low-dose group, notoginseng total
saponin preparation middle-dose group, notoginseng total
saponin preparation high-dose group on average. They are treated by
intravenous injection of test substance solutions containing 0.4%
Evans blue (EB). 30 min later, scores of ear blue staining and quantitation of ear EB exudation were recorded. Another two experiment were repeated in the same way excluding EB, just to. detect the related
cytokines in serum using ELISA. We found that the scores of pseudoanaphylactoid reaction in notoginseng total
saponin preparation injection middle-dose group and high-dose group was evidently higher than that in control group, suggesting that notoginseng total
saponin preparation injection may be can lead to pseadoanaphylactoid reaction. HE staining showed that pseadoanaphylactoid reaction induced by notoginseng total
saponin preparation injection is related to
inflammation.
Histamine,
VEGF and TNF-α levels in notoginseng total
saponin preparation middle-dose group and high-dose group significantly increased (P < 0.05, P < 0.01) than control group and showed a dose-dependent manner as well as consistent with the degree of ear blue
dye. While
IL-6 and
IL-10 content did not increase significantly in notoginseng total
saponin preparation low-dose group and middle-dose group, but they significantly higher than control group (P < 0.05, P < 0.01) when it increased to quadrupe clinical concentrations, eight times of the clinical dose. So pseadoanaphylactoid reaction caused by notoginseng total
saponin preparation may be related to
histamine,
VEGF, TNF-α, and it is possible that
IL-6 and
IL-10 can play a role when pseadoanaphylactoid reaction achieve a certain high degree.