[Studying the tumor growth inhibitory effect of modified GnRH-III-anthracycline bioconjugates in subcutaneous vs. orthotopic models in vivo].

Abstract

Targeted tumor therapy is a perspective procedure to specifically destroy the cancer tissues with eliminating or at least decreasing the side effects of anticancer drugs. For this purpose the drug molecule is attached to a targeting moiety (e.g. peptide hormones) that recognizes tumor specific or overexpressed receptors on cancer cells. The in vitro cytostatic or cytotoxic assays do not give proper information whether the tumor growth inhibitory effect of the conjugate is better than the activity of the free drug. Only in vivo studies are adequate to answer this question. However, the selection of the appropriate tumor model is important to eliminate the false positive results. In our studies a gonadotropin-releasing hormone analog (GnRH-III) was applied as targeting moiety in drug conjugates. The in vivo antitumor activity of these conjugates was investigated on mice bearing subcutaneously or orthotopically szigdeveloped tumors. The subcutaneously implanted tumor model which is isolated from its surroundings may provide false results in tumor growth inhibition. In contrast, the orthotopically developed tumor is a better model representing appropriate anatomical and clinical status of cancer. Therefore, the orthotopical colon cancer developed in our laboratory is a suitable model for the study of the antitumor activity of the conjugates prepared for targeted tumor therapy.

• Authors: Bence Kapuvári, Ákos Schulcz, Rózsa Hegedüs, Ildikó Szabó, Marilena Manea, Borbála Vincze, József Tóvári, Alexandra Gacs, Miguel Tejeda, Dezsõ Gaál, Gábor Mezõ
• Journal: Magyar onkologia (Magy Onkol) Vol. 59 Issue 4 Pg. 310-8 (Dec 2015) ISSN: 0025-0244 [Print] Hungary
• Vernacular Title: Módosított GnRH-III-antraciklin biokonjugátumok daganatnövekedést gátló hatásának tanulmányozása in vivo szubkután vs. ortotopikus rendszerekben.
• PMID: 26665191 (Publication Type: English Abstract, Journal Article)