Detection of ras gene alterations and ras proteins in colorectal cancer.

Abstract

DNA extracted from 31 primary colorectal carcinomas was analyzed for the presence of ras gene amplification and mutations. Nine carcinomas had Ha-ras amplification and seven Ki-ras amplification. Nine carcinomas had codon 12 Ki-ras mutations. Immunohistochemical staining for ras proteins revealed a normal membrane association in normal mucosa and benign polyps but an abnormal cytoplasmic distribution in carcinomas. Amplification, mutations, and immunohistochemical staining were independent of histologic differentiation, Dukes' stage, or DNA ploidy status. This study demonstrates that abnormalities of ras genes are a common finding in colorectal carcinomas. They are potentially important biologic changes associated with malignancy, although they do not appear to be related to clinical behavior.

• Authors: N Salhab, D J Jones, J L Bos, A Kinsella, P F Schofield
• Journal: Diseases of the colon and rectum (Dis Colon Rectum) Vol. 32 Issue 8 Pg. 659-64 (Aug 1989) ISSN: 0012-3706 [Print] United States
• PMID: 2666051 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Proto-Oncogene Proteins
• HRAS protein, human
• Proto-Oncogene Proteins p21(ras)

Topics

• Adult
• Aged
• Aged, 80 and over
• Carcinoma (analysis)
• Colonic Neoplasms (analysis)
• DNA, Neoplasm (analysis)
• Female
• Gene Amplification
• Genes, ras
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Mutation
• Nucleic Acid Hybridization
• Ploidies
• Proto-Oncogene Proteins (analysis)
• Proto-Oncogene Proteins p21(ras)
• Rectal Neoplasms (analysis)