Systematic evaluation of self-adjuvanting lipopeptide nano-vaccine platforms for the induction of potent CD8(+) T-cell responses.

Abstract

AIM:

Systematically evaluate lipid core peptide vaccine delivery platforms to identify core features promoting strong CD8(+) T-cell responses.

MATERIALS & METHODS:

Three different self-adjuvanting lipid core peptide nanovaccines each comprising four copies of the dominant ovalbumin CD8(+) T-cell epitope and varying in the utilization of a polylysine or glucose core with 2-amino-hexadecanoic acid (C16) or 2-amino-dodecanoic acid (C12) lipids were synthesized. Vaccines were tested for ability to induce CD8(+) T-cell responses and inhibit tumor growth in vivo.

RESULTS:

The construct utilizing C12 lipids and polylysine core induced very robust effector T cells shown to have in vivo effector capability as demonstrated by in vivo cytotoxicity and ability to inhibit tumor growth as well as modulation of dendritic cell activation.

CONCLUSION:

The C12 polylysine platform was an effective configuration for induction of potent CD8(+) T-cell responses.

• Authors: Simon H Apte, Rachel J Stephenson, Pavla Simerska, Penny L Groves, Salwa Aljohani, Sharareh Eskandari, Istvan Toth, Denise L Doolan
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 11 Issue 2 Pg. 137-52 (Jan 2016) ISSN: 1748-6963 [Electronic] England
• PMID: 26653407 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cancer Vaccines
• Lauric Acids
• Lipopeptides
• Nanocapsules
• Polylysine
• 12-aminododecanoic acid

Topics

• Animals
• CD8-Positive T-Lymphocytes (drug effects, immunology)
• Cancer Vaccines (administration & dosage, chemistry)
• Lauric Acids (chemistry)
• Lipopeptides (administration & dosage, chemistry)
• Mice
• Mice, Inbred C57BL
• Nanocapsules (chemistry, ultrastructure)
• Neoplasms, Experimental (immunology, therapy)
• Particle Size
• Polylysine (chemistry)
• Treatment Outcome